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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Laboratory Operations- Quality and safety in the clinical lab
  • 1. Quality control and assurance
    • 2. Laboratory safety and regulations
      Topic 2: Urinalysis and Body Fluids- Fluid analysis and interpretation
      • 1. CSF and other body fluids
        • 2. Urine chemical and microscopic examination
          Topic 3: Clinical Chemistry- Metabolic and analytical testing
          • 1. Endocrine and metabolic panels
            • 2. Enzymes and electrolytes
              Topic 4: Hematology- Blood cell analysis and disorders
              • 1. CBC and differential interpretation
                • 2. Anemias and leukemias overview
                  Topic 5: Blood Banking / Immunohematology- Blood group systems and antibody identification
                  • 1. Crossmatching procedures
                    • 2. ABO and Rh systems
                      Topic 6: Microbiology- Clinical microbiological techniques
                      • 1. Bacteriology and organism identification
                        • 2. Culture and sensitivity testing

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. This Gram stain is under-decolorized
                          This microscopic field is representative of other fields that were observed on a Gram-stained smear. Which of the following describes the quality of the smear?

                          A) Over-decolorized
                          B) Sufficiently decolorized
                          C) Under-decolorized


                          2. The renal threshold is the maximum amount of a substance that the kidney can prevent from entering into the urine.
                          UA & body fluids
                          The renal threshold is best described as:

                          A) Concentration at which kidney failure begins
                          B) Concentration at which reabsorption first occurs
                          C) Concentration at which a substance in the blood spills into urine
                          D) Concentration at which kidney can no longer filter the blood


                          3. A casual blood glucose >/= 200 mg/dL on a patient with symptoms and an A1C >/= 6.5% meet diagnostic criteria for diabetes.
                          Chemistry
                          A physician is evaluating a 45-year-old obese male for diabetes and orders a plasma glucose at time of evaluation and a HbA1C one week later. The patient has a family history of diabetes and currently exhibits symptoms of diabetes.
                          What would be the best course of action if these are the blood glucose results?
                          Casual Blood Glucose: 208 mg/dL
                          HbA1C one week later: 7.2%

                          A) The patient is at risk for diabetes (pre-diabetic); monitor for diabetes with yearly FPG levels
                          B) Order a C-peptide for diagnosis
                          C) Order insulin levels; if decreased, diagnose diabetes
                          D) Patient meets criteria for diagnosis of diabetes


                          4. Transmission-based precautions isolation categories include all of the following except:

                          A) airborne.
                          B) droplet.
                          C) blood and body fluids.
                          D) contact.


                          5. In radioimmunassay, a fixed concentration of labeled antigen is incubated with a constant amount of antiserum such that the concentration of antigen binding sites on the antibody is limiting. If unlabeled antigen is added to this system, there is competition between labeled antigen and unlabeled antigen for the limited and constant number of binding sites on the antibody, and thus the amount of labeled antigen bound to antibody will decrease as the concentration of unlabeled antigen increases.
                          Which of the following choices is correct when describing the principles of competitive radioimmunoassay procedures?

                          A) antibody will react directly with radioactive labeled immunoglobulin
                          B) antibody will react with labeled antigen only
                          C) antibody will react equally with labeled and unlabeled antigen
                          D) antibody will react preferentially with the labeled antigen
                          E) antibody will react preferentially with the unlabeled antigen


                          Solutions:

                          Question # 1
                          Answer: C
                          Question # 2
                          Answer: C
                          Question # 3
                          Answer: D
                          Question # 4
                          Answer: C
                          Question # 5
                          Answer: C

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